Chemistry 104

Chemistry 451

Lecture #4: Recombinant DNA technology (continued); and Emerging Technologies

Read:

Pg. 67-75

The RNA message is sometimes edited (animation explains restriction mapping)

Optional:

Guided Exploration 2 (CD)

DNA animations (shown in class: southern blotting, DNA detective, PCR)

HW (Due Monday at end of class)

Three potential topics for "Thinking Like a Biochemist" assignment

(This replaces assignment for group rosters listed on the syllabus. I will help organize groups based on topics of interest. If you already have a group or have chosen a topic, identify group members or individuals you would like to work with and abstracts of papers you are considering).

pg. 76 (6, 7, 9, 10, 11)

Objectives:

Understand DNA fingerprinting, recombinant clones and PCR
Be able to piece together a sequence of DNA given a restriction map.