Lecture #14: Enzyme Kinetics: Michaelis-Menten

Read: pg. 322-332

Optional Reading: Enzymes and how to interpret plots of vo vs. [S]

1. Write the rate equation for a first order reaction and determine if a reaction is first order from a plot of ln[A] vs time.
2. Be able to derive the Michaelis-Menten equation (v _o=V_max[S]/(K_m + [S]).
3. Explain why assumptions of equilibrium and steady state are necessary to derive AND interpret a plot of the initial velocity (v _o) vs. [S].
4. Know that V_max is achieved when enzyme is saturated with substrate and that K_m is a measure of affinity of enzyme for substrate. (plot of v_o vs. [S] is hyperbolic).
5. Be able to write the Lineweaver-Burk (double-reciprocal) equation and describe its features.
6. Be able to determine Km and V_max if given [S] and v_o (sample calculation on pg. 331).